Composite

Part:BBa_K2129002:Design

Designed by: Maxwell Saal   Group: iGEM16_CU-Boulder   (2016-10-13)


Mutant EutS with Mid expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This locus was selected based on predictive modelling of EutS superstructure to incorporate at a locus where a phenylalanine-4'-azobenzene non-canonical amino acid might be able to hinder superstructure formation when under cis or trans conditions.


Source

EutS derived from K2129001, mutated with site directed mutagenesis (QuikChange).

References